Oligo-dT anchored cDNA-SRAP and cDNA-SCoT aided identification of transcripts differentially expressed during the early stages of recovery of resurrection plant <i>Haberlea rhodopensis</i> Friv. from freezing-induced desiccation

Resurrection plants are a unique group of flora showing an extraordinary ability to survive extreme water shortages. Among them, the Bulgarian endemic plant Haberlea rhodopensis Friv. has been used as model study mechanisms underlying desiccation tolerance induced by and low temperature stress. Although extensive transcriptome data, including two RNAseq experiments, available for drought recovery, gene expression during recovery after freezing-induced (RAF) remains largely unexplored. In this we performed differential screening using new approach, based on oligo-dT anchored cDNA-SRAP (copy DNA sequence-related amplified polymorphism) modified cDNA-SCoT start codon targeted) assays provide information genes involved in stress H. rhodopensis. The putative functions 19 identified transcript derived fragments were established searching homology with known sequences public databases 10 them early stages was confirmed quantitative reverse transcription polymerase chain reaction. Some have response abiotic other species resurrection plants, which confirms effectiveness approach present study. results will contribute obtaining defence process rhodopensis, especially at is most vulnerable period plants.